Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis

ABSTRACT: The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis assessed if the histopathological examination polymerase chain reaction (PCR) were effective studies like this. Trachea, liver, lung collected from 30 chickens with suspected that has been inspection line. samples analyzed PCR to simultaneously identify two (iss tsh genes) testing. efficiently genotypically characterize E. isolates, where iss found three birds simultaneously. detected a predominance of heterophils mononuclear cells trachea (100%), (90%), liver (13.3%). was organ practically no alteration diagnosed. results multiplex indicate great potential approach characterization isolates. Unspecific identification did not occur, thus making it necessary use technologies prevention this agent aviaries poultry abattoirs.